R. Alm, A. Ekefjärd, M. Krogh, J. Häkkinen, C. Emanuelsson
Proteomic variation is larger within than between strawberry varieties
Submitted.

Abstract: In search for a strawberry (Fragaria ananassa) with low allergen content, we determined the proteomic variation within and between different varieties. Proteomics data were generated by DIGE and proteins identified with MALDI-MS/MS. The amount of the allergen Fra a 1 was found to be consistently low in several different colourless (white) cultivars. The amount of allergen varied between different red strawberry varieties (CV=39%). The variation was at the same level, or even slightly larger, due to different growth conditions (CV=43%). For 153 other proteins, the biological variation was more affected by different growth conditions than by different varieties (mean CV=52% and 43%, respectively) due to variation in a subset of proteins. The finding that allergen variation due to growth condition differences is as large or even larger than variety differences has important implications for efforts to obtain strawberries with low allergen content.

LU TP 07-06