R. Alm, A. Ekefjärd, M. Krogh, J. Häkkinen, C. Emanuelsson
Proteomic variation is larger within than
between strawberry varieties
Submitted.
Abstract:
In search for a strawberry (Fragaria ananassa) with low
allergen content, we determined the proteomic variation within
and between different varieties. Proteomics data were generated
by DIGE and proteins identified with MALDI-MS/MS. The amount of
the allergen Fra a 1 was found to be consistently low in several
different colourless (white) cultivars. The amount of allergen
varied between different red strawberry varieties (CV=39%). The
variation was at the same level, or even slightly larger, due to
different growth conditions (CV=43%). For 153 other proteins,
the biological variation was more affected by different growth
conditions than by different varieties (mean CV=52% and 43%,
respectively) due to variation in a subset of proteins. The
finding that allergen variation due to growth condition
differences is as large or even larger than variety differences
has important implications for efforts to obtain strawberries
with low allergen content.
LU TP 07-06