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<body bgcolor = "#FFFFCC"><basefont face = "Arial"> |
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<h1>LEM: Linear Expression Map</h1><h2>Parameter Information</h2> |
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<hr size = 10> |
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These initialization parameters are used to direct MeV to information about the |
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nature of the arrayed spots and the sampled genome. The parameters that are |
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selected in this dialog will determine the Linear Expression Map that is produced. |
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Incorrect selections will produce maps that are not correct. If a map looks |
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unusual you can check the entered parameters in the <i>LEM Mapping Summary</i> |
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node on the result tree. |
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<h2>Locus Identifier Selection</h2> |
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This menu provides a list all spot annotation fields in MeV. |
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Select the annotation field that should be used to map each spot |
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to an associated locus. |
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<h2>Coordinate Data Selections</h2> |
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<b><i>Use Coordinate File</i></b><br> |
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This option, when selected, indicates that chromosome location and |
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coordinate information will come from an auxilliary file. The format of this |
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file is shown in the manual section for LEM. The coordinate file is a text |
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tab delimited format file that contains no header and 3 or 4 columns. |
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<br><center> < locus_id > [chromosome_id] < 5'_end > < 3'_end > </center><br> |
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The second column, chromosome_id is only needed if the organisim under |
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study has multple chromosomes.<br><br> |
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<b><i>Multiple Chromosomes</i></b><br> |
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If selected, this option indicates that there are spots from loci on |
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multiple chromosomes on the array. When selected the information to map |
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loci to chromosomes is expected to be in the input file or within an |
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annoation field if the coordinate file is not used. If not using a file one |
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should indicate which field of spot annotation corresponds to chromosomal |
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membership information. (mapping spots to chromosomes)<br><br> |
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<b><i>Coordinate Information</i></b><br> |
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If not using a coordinate file, these menus are used to |
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select annotation fields that indicate 5' and 3' ends of the locus.<br> |
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</basefont> |
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</body> |
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</html> |