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<html> |
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<body bgcolor = "#FFFFCC"><basefont face = "Arial"> |
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<h1>Color Scale Limits Dialog</h1><h2>Information</h2> |
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<hr size = 10> |
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<h2> Gradient Style </h2> |
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The gradient style controls are used to select to use either |
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a double gradient such as green-black-red or a single gradient |
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such as black-red. The double gradient is most often used for |
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two dye array systems in which the values are log<sub>2</sub> |
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ratios. In this case the mid-point is kept at 0.0 so that |
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spots where the two channels have equal expression (log<sub>2</sub>(A/B) = 0) are |
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depicted as black and spots where the expression is unequal will |
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tend to be colored more toward the extremes. The double gradient |
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might also be useful for single dye systems where a reference |
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or median value is used to generate a log<sub>2</sub>(I/Ref). |
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<br><br> |
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The single gradient option is useful for single channel data |
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where data falls over a continuum and there is no clear rationale |
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to separate the data into two distinct color regions. |
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<h2> Color Range Selection </h2> |
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This section of the dialog is used to indicate the limits for the |
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color scale and an optional "mid-point" value. The single gradient mode |
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only requires endpoints while the double gradient mode provides |
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for selection of a mid-point value. For two dye systems the mid-point |
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should generally be left at zero so that spots with equal expression |
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in the two channels are displayed as black. |
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<br><br> |
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Values that exceed the limits that are set will be displayed as the |
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saturated endpoint color. The range of the data is given in parentheses |
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next to the limit input boxes to assist in initial limit selections. |
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The <i>Update Limits</i> button in this panel will apply the current |
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limits to the display in MeV and will update the |
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<i>Color Saturation Statistics</i> panel and <i>Gradient and Limits Preview</i> panel. |
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<h2> Color Saturation Statistics </h2> |
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This section of the dialog reports the number of elements that are |
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beyond the limits defined by the color scale. These off-scale |
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elements are displayed as the endpoint color of gradient closest |
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to the element's value. The statistics are calculted using all |
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spots that are not missing values and have not been filtered out of |
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the main data set by an applied filter. |
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<h2> Limits and Gradient Preview </h2> |
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This panel presents the current gradient style and the selected range limits. |
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<br> |
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</basefont> |
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</body> |
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</html> |