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<%@ page |
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pageEncoding="UTF-8" |
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session="false" |
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import="net.sf.basedb.core.User" |
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import="net.sf.basedb.core.DbControl" |
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import="net.sf.basedb.core.SessionControl" |
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import="net.sf.basedb.core.Application" |
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import="net.sf.basedb.core.Extract" |
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import="net.sf.basedb.core.BioMaterial" |
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import="net.sf.basedb.core.MeasuredBioMaterial" |
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import="net.sf.basedb.core.BioMaterialEventSource" |
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import="net.sf.basedb.core.BioPlate" |
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import="net.sf.basedb.core.BioWell" |
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import="net.sf.basedb.core.PermissionDeniedException" |
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import="net.sf.basedb.core.Project" |
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import="net.sf.basedb.core.ItemQuery" |
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import="net.sf.basedb.core.query.Restrictions" |
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import="net.sf.basedb.core.query.Hql" |
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import="net.sf.basedb.util.Values" |
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import="net.sf.basedb.util.formatter.WellCoordinateFormatter" |
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import="net.sf.basedb.util.extensions.Extension" |
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import="net.sf.basedb.clients.web.Base" |
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import="net.sf.basedb.clients.web.util.HTML" |
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import="net.sf.basedb.clients.web.extensions.ExtensionsControl" |
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import="java.util.Arrays" |
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import="java.util.Enumeration" |
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import="java.util.Collections" |
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%> |
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<%@ taglib prefix="base" uri="/WEB-INF/base.tld" %> |
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<% |
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final SessionControl sc = Base.getExistingSessionControl(request, true); |
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final String ID = sc.getId(); |
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final String home = ExtensionsControl.getHomeUrl("net.sf.basedb.meludi"); |
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final String root = request.getContextPath(); |
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DbControl dc = null; |
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try |
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{ |
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dc = sc.newDbControl(); |
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final Extension meludi = ExtensionsControl.get(dc).getExtension("net.sf.basedb.meludi"); |
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final User user = User.getById(dc, sc.getLoggedInUserId()); |
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Project activeProject = Project.getById(dc, sc.getActiveProjectId()); |
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String projectName = activeProject.getName(); |
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|
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String submitInfoJsonStr = Values.getStringOrNull(request.getParameter("hiddenSubmitInfo")); |
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Integer numItems = Values.getInteger(request.getParameter("hiddenNumItems"), null); |
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%> |
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<base:page id="protocol" type="iframe" favicon="<%=home+"/images/report.png"%>" noskin="true"> |
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<base:head |
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scripts="~../meludi-2.js,~libprep_dilution_protocol.js" |
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styles="~../css/meludi-2.css,~../css/printable.css" |
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> |
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|
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<style> |
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h1 |
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{ |
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margin-top: 1em; |
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} |
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|
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h2 |
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{ |
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margin-bottom: 0; |
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font-size: 1.2em; |
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} |
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|
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table.protocolheader |
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{ |
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width: 100%; |
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border: 1px solid #000000; |
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margin-bottom: 1em; |
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/* |
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border-collapse: collapse; |
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*/ |
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} |
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table.protocolheader > tbody > tr |
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{ |
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vertical-align: middle; |
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height: 2.5em; |
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max-height: 2.5em; |
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border-bottom: 1px solid #000000; |
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border-top: 1px solid #000000; |
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} |
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table.protocolheader > tbody > tr > th |
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{ |
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text-align: left; |
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font-size: 1em; |
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background-color: #F0F0F0; |
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padding: 4px; |
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border-left: 1px solid #000000; |
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border-right: 1px solid #000000; |
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} |
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table.protocolheader > tbody > tr > th.subprompt |
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{ |
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text-align: right; |
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font-weight: normal; |
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} |
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table.protocolheader > tbody > tr > td |
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{ |
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text-align: left; |
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font-size: 1em; |
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padding: 4px; |
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} |
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|
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.lowvolume |
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{ |
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color: #C80000; |
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} |
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|
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.colorbox |
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{ |
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display: inline-block; |
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width: 0.75em; |
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height: 0.75em; |
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border: 1px solid #000000; |
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margin-left: 0em; |
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margin-right: 0.5em; |
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} |
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.patient-legend |
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{ |
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width: 8em; |
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text-align: center !important; |
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} |
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|
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.allcases-legend |
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{ |
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width: 8em; |
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text-align: center !important; |
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} |
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|
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.green |
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{ |
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background-color: #D8F8D8; |
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} |
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.green.darker |
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{ |
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background-color: #A0F8A0; |
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} |
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|
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.blue |
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{ |
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background-color: #D8E8FF; |
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} |
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.blue.darker |
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{ |
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background-color: #B8B8FF; |
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} |
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|
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.yellow |
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{ |
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background-color: #FFFFD8; |
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} |
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.yellow.darker |
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{ |
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background-color: #FFFFA0; |
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} |
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|
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.red |
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{ |
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background-color: #FFC8C8; |
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} |
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.red.darker |
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{ |
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background-color: #F8A0A0; |
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} |
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|
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#allprepLabTrackingProtocolHeader |
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{ |
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font-size: 1.2em; |
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} |
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|
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.reagent |
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{ |
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width: 70%; |
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height: 80%; |
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font-size: 0.9em; |
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} |
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|
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.reagentheader |
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{ |
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width: 30%; |
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height: 80%; |
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font-size: 0.9em; |
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} |
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</style> |
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</base:head> |
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<base:body> |
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<div class="paper"> |
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<form name="meludi" id="wizard"> |
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|
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<div id="page-data" class="datacontainer" |
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data-submitinfo="<%=HTML.encodeTags(submitInfoJsonStr)%>" |
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data-numitems="<%=numItems%>" |
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data-projectname="<%=projectName%>" |
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data-meludiversion="<%=meludi.getAbout().getVersion()%>" |
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></div> |
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|
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<div id="wizard-status"></div> |
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|
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<div id="all-protocol" style="display: none;"> |
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|
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<div id="print-instructions" class="noprint fullwidth"> |
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<base:button id="print-button" image="<%=home+"/images/print.png"%>" title="Print…" /> |
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<span id="printNote"> |
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<b>Note!</b> |
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For better printing reduce margins to about <i>5mm</i> and set page orientation |
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to <i>portrait</i>. |
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</span> |
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<br clear="all"> |
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</div> |
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|
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<h1 id="libraryPreparationDilutionProtocolHeader">Dilution protocol for library preparation v1.0<span class="meludi">MeLuDI <%=meludi.getAbout().getVersion() %></span></h1> |
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|
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<!-- Table with two columns; left for start plate name, right for dilution protocol date --> |
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<table class="protocolheader"> |
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<!-- |
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<tr> |
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<th style="max-height: 1.5em; font-size: 0.9em;">Start plate</th> |
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<td><span id="startplateid" style="max-height: 1.5em; font-size: 0.9em;"></span></td> |
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<th style="max-height: 1.5em; font-size: 0.9em;" id="protocolDateName">Dilution protocol date</th> |
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<td><span id="dilutionProtocolDate" style="max-height: 1.5em; font-size: 0.9em;"></span></td> |
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</tr> |
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--> |
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<tr> |
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<th style="max-height: 1.5em; font-size: 0.9em;">Start plate</th> |
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<td><span id="startplateid" style="max-height: 1.5em; font-size: 0.9em;"></span></td> |
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<th style="max-height: 1.5em; font-size: 0.9em;" id="protocolDateName">Dilution protocol date</th> |
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<td><span id="dilutionProtocolDate" style="max-height: 1.5em; font-size: 0.9em;"></span></td> |
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<th style="max-height: 1.5em; font-size: 0.9em;" id="projectNameTitle">Project</th> |
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<td><span id="projectnameid" style="max-height: 1.5em; font-size: 0.9em;"></span></td> |
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</tr> |
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</tbody> |
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</table> |
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<BR> |
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10µl should be taken from the solution to each aliquot (FPA and FPB) |
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<BR> |
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<table class="protocolheader" border="1"> |
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<tr id="extractionlist"> |
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<th style="font-size: 0.9em; width: 12%;">Extract</th> |
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<th style="font-size: 0.9em; width: 10%;">DNA plate pos.</th> |
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<th style="font-size: 0.9em; width: 3%;">FPA pos.</th> |
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<th style="font-size: 0.9em; width: 3%;">FPB pos.</th> |
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<th style="font-size: 0.9em; width: 7%;">Volume DNA (µl)</th> |
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<th style="font-size: 0.9em; width: 7%;">Volume water (µl)</th> |
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<th style="font-size: 0.9em; width: 10%;">Solution volume (µl)</th> |
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<th style="font-size: 0.9em; width: 10%;">Dilution factor</th> |
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<th style="font-size: 0.9em; width: 10%;">ΔCt</th> |
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<th style="font-size: 0.9em; width: 10%;">DNA conc. Qubit (ng/µl)</th> |
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<th style="font-size: 0.9em; width: 18%;">Remark</th> |
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</tr> |
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<% |
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for (int itemNo = 0; itemNo < numItems; ++itemNo) |
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{ |
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%> |
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<tr> |
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<td style="font-size: 0.9em; width: 12%; border-right: 1px solid #000000;"><div id="extract.<%=itemNo%>" >Extract <%=itemNo%></div></td> |
3590 |
11 Nov 15 |
olle |
261 |
<td style="font-size: 0.9em; width: 10%; text-align: center; border-right: 1px solid #000000;"><div id="dnaStorageBox.<%=itemNo%>" >DNA position <%=itemNo%></div></td> |
3590 |
11 Nov 15 |
olle |
262 |
<td style="font-size: 0.9em; width: 3%; text-align: center; border-right: 1px solid #000000;"><div id="fpaPos.<%=itemNo%>" >DNA volume <%=itemNo%></div></td> |
3590 |
11 Nov 15 |
olle |
263 |
<td style="font-size: 0.9em; width: 3%; text-align: center; border-right: 1px solid #000000;"><div id="fpbPos.<%=itemNo%>" >Water volume <%=itemNo%></div></td> |
3590 |
11 Nov 15 |
olle |
264 |
<td style="font-size: 0.9em; width: 7%; text-align: center; border-right: 1px solid #000000;"><div id="dnaVolume.<%=itemNo%>" >DNA volume <%=itemNo%></div></td> |
3590 |
11 Nov 15 |
olle |
265 |
<td style="font-size: 0.9em; width: 7%; text-align: center; border-right: 1px solid #000000;"><div id="waterVolume.<%=itemNo%>" >Water volume <%=itemNo%></div></td> |
3590 |
11 Nov 15 |
olle |
266 |
<td style="font-size: 0.9em; width: 10%; text-align: center; border-right: 1px solid #000000;"><div id="solutionVolume.<%=itemNo%>" >Solution volume <%=itemNo%></div></td> |
3590 |
11 Nov 15 |
olle |
267 |
<td style="font-size: 0.9em; width: 10%; text-align: center; border-right: 1px solid #000000;"><div id="dilutionFactor.<%=itemNo%>" >Dilution factor <%=itemNo%></div></td> |
3590 |
11 Nov 15 |
olle |
268 |
<td style="font-size: 0.9em; width: 10%; text-align: center; border-right: 1px solid #000000;"><div id="deltaCt.<%=itemNo%>" >Delta-Ct <%=itemNo%></div></td> |
3590 |
11 Nov 15 |
olle |
269 |
<td style="font-size: 0.9em; width: 10%; text-align: center; border-right: 1px solid #000000;"><div id="dnaConc.<%=itemNo%>" >DNA conc. <%=itemNo%></div></td> |
3590 |
11 Nov 15 |
olle |
270 |
<td style="font-size: 0.9em; width: 18%; border-right: 1px solid #000000;"><div id="remark.<%=itemNo%>" >Remark <%=itemNo%></div></td> |
3590 |
11 Nov 15 |
olle |
271 |
</tr> |
3590 |
11 Nov 15 |
olle |
272 |
<% |
3590 |
11 Nov 15 |
olle |
273 |
} |
3590 |
11 Nov 15 |
olle |
274 |
%> |
3590 |
11 Nov 15 |
olle |
275 |
</table> |
3590 |
11 Nov 15 |
olle |
276 |
<div class="pagebreak"></div> |
3590 |
11 Nov 15 |
olle |
277 |
|
3590 |
11 Nov 15 |
olle |
278 |
</div> |
3590 |
11 Nov 15 |
olle |
279 |
</form> |
3590 |
11 Nov 15 |
olle |
280 |
</div> |
3590 |
11 Nov 15 |
olle |
281 |
</base:body> |
3590 |
11 Nov 15 |
olle |
282 |
</base:page> |
3590 |
11 Nov 15 |
olle |
283 |
<% |
3590 |
11 Nov 15 |
olle |
284 |
} |
3590 |
11 Nov 15 |
olle |
285 |
finally |
3590 |
11 Nov 15 |
olle |
286 |
{ |
3590 |
11 Nov 15 |
olle |
287 |
if (dc != null) dc.close(); |
3590 |
11 Nov 15 |
olle |
288 |
} |
3590 |
11 Nov 15 |
olle |
289 |
%> |