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29 Aug 22 |
nicklas |
Bootstrap: library |
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29 Aug 22 |
nicklas |
From: library/default/rockylinux:9.0 |
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29 Aug 22 |
nicklas |
3 |
|
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29 Aug 22 |
nicklas |
%post |
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29 Aug 22 |
nicklas |
yum -y install glibc-langpack-en libxcrypt-compat |
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29 Aug 22 |
nicklas |
yum -y install findutils wget unzip tar bzip2 hostname |
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29 Aug 22 |
nicklas |
7 |
|
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29 Aug 22 |
nicklas |
## Minconda 4.12 with Python 3.9 |
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29 Aug 22 |
nicklas |
mkdir -p /download |
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29 Aug 22 |
nicklas |
wget https://repo.anaconda.com/miniconda/Miniconda3-py39_4.12.0-Linux-x86_64.sh -O /download/miniconda.sh |
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29 Aug 22 |
nicklas |
11 |
|
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29 Aug 22 |
nicklas |
bash /download/miniconda.sh -b -p /miniconda |
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29 Aug 22 |
nicklas |
PATH=/miniconda/bin:$PATH |
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29 Aug 22 |
nicklas |
conda config --add channels defaults |
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29 Aug 22 |
nicklas |
conda config --add channels bioconda |
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29 Aug 22 |
nicklas |
conda config --add channels conda-forge |
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29 Aug 22 |
nicklas |
17 |
|
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29 Aug 22 |
nicklas |
## Stringtie |
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29 Aug 22 |
nicklas |
conda install -y \ |
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29 Aug 22 |
nicklas |
stringtie=2.2.1 |
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29 Aug 22 |
nicklas |
21 |
|
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29 Aug 22 |
nicklas |
## Cleanup |
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29 Aug 22 |
nicklas |
rm -rf /download |
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29 Aug 22 |
nicklas |
conda clean -y --all |
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29 Aug 22 |
nicklas |
yum clean all |
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29 Aug 22 |
nicklas |
26 |
|
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29 Aug 22 |
nicklas |
%environment |
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29 Aug 22 |
nicklas |
export PATH=/miniconda/bin:$PATH |
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29 Aug 22 |
nicklas |
export STRINGTIE=/miniconda/bin/stringtie |
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29 Aug 22 |
nicklas |
export PREPDE=/miniconda/bin/prepDE.py |
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29 Aug 22 |
nicklas |
31 |
|
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29 Aug 22 |
nicklas |
%test |
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29 Aug 22 |
nicklas |
echo "Host : `hostname 2>&1`" |
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29 Aug 22 |
nicklas |
echo "OS : `cat /etc/os-release | grep PRETTY_NAME | cut -d '"' -f 2`" |
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29 Aug 22 |
nicklas |
echo "Stringtie : `stringtie --version 2>&1`" |
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29 Aug 22 |
nicklas |
echo "Miniconda : `conda --version 2>&1`" |
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29 Aug 22 |
nicklas |
echo "Python : `python --version 2>&1`" |
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29 Aug 22 |
nicklas |
38 |
|
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29 Aug 22 |
nicklas |
%labels |
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29 Aug 22 |
nicklas |
PipelineVersion 2023.v1 |
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29 Aug 22 |
nicklas |
Author Nicklas Nordborg |
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29 Aug 22 |
nicklas |
42 |
|
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29 Aug 22 |
nicklas |
%help |
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29 Aug 22 |
nicklas |
A container with the tools that are needed for the StringTie |
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29 Aug 22 |
nicklas |
gene expression analysis. To display versions of installed |
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29 Aug 22 |
nicklas |
programs use: |
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29 Aug 22 |
nicklas |
47 |
|
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29 Aug 22 |
nicklas |
singularity test <container.sif> |