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<%@ page |
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pageEncoding="UTF-8" |
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session="false" |
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import="net.sf.basedb.core.User" |
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import="net.sf.basedb.core.DbControl" |
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import="net.sf.basedb.core.SessionControl" |
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import="net.sf.basedb.core.Application" |
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import="net.sf.basedb.clients.web.Base" |
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import="net.sf.basedb.clients.web.util.HTML" |
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import="net.sf.basedb.clients.web.extensions.ExtensionsControl" |
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%> |
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<%@ taglib prefix="base" uri="/WEB-INF/base.tld" %> |
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<%@ taglib prefix="p" uri="/WEB-INF/path.tld" %> |
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<% |
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final SessionControl sc = Base.getExistingSessionControl(request, "net.sf.basedb.reggie", true); |
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final String ID = sc.getId(); |
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final float scale = Base.getScale(sc); |
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final String home = ExtensionsControl.getHomeUrl("net.sf.basedb.reggie"); |
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DbControl dc = null; |
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try |
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{ |
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dc = sc.newDbControl(); |
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final User user = User.getById(dc, sc.getLoggedInUserId()); |
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%> |
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<base:page type="default" > |
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<base:head |
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scripts="~../reggie-2.js,~align_start.js" |
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styles="path.css,~../css/reggie-2.css" |
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/> |
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<base:body> |
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|
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<p:path><p:pathelement |
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title="Reggie" href="<%="../index.jsp?ID="+ID%>" |
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/><p:pathelement title="Start Tophat and Cufflinks legacy pipeline" |
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/></p:path> |
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|
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<div class="content"> |
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<form name="reggie" id="wizard" class="wizard"> |
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|
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<div class="step auto-hide" id="step-1"> |
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<div class="step-no">1</div> |
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<div class="step-title">Select merged sequences</div> |
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<div class="step-content"> |
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|
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<table class="step-form"> |
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<tr class="align-top"> |
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<td class="prompt">Merged sequences</td> |
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<td class="input"> |
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<select class="required yellow-label-support" multiple size="30" |
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name="mergedSequences" id="mergedSequences"></select> |
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|
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<base:buttongroup style="margin-top: 0.5em;"> |
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<base:button title="Select manually…" id="btnSelectMergedSequences" /> |
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</base:buttongroup> |
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</td> |
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<td class="status" id="mergedSequences.status"></td> |
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<td class="help"><span id="mergedSequences.message" class="message"></span> |
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Select the sequences that should be aligned. The list contains merged sequences that |
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from the <b>Legacy pipeline</b> item list (max 250).<br><br> |
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Items marked with <b>[R]</b> have been marked for re-alignment due to a failure of |
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a previous alignment. |
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</td> |
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</tr> |
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</table> |
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|
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</div> |
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</div> |
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|
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|
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<div class="step" id="step-2"> |
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<div class="step-no">2</div> |
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<div class="step-title">Masking and alignment information</div> |
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<div class="step-content"> |
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|
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<table class="step-form"> |
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<tbody> |
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<tr class="section-header"> |
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<td colspan="4">Masking</td> |
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</tr> |
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<tr> |
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<td class="subprompt">Protocol</td> |
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<td class="input"> |
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<select name="maskingProtocol" id="maskingProtocol"></select> |
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</td> |
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<td class="status" id="maskingProtocol.status"></td> |
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<td class="help"> |
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<span id="maskingProtocol.message" class="message"></span> |
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Select the protocol used for filtering. |
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</td> |
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</tr> |
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<tr> |
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<td class="subprompt">Software</td> |
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<td class="input"> |
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<select name="maskingSoftware" id="maskingSoftware"></select> |
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</td> |
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<td class="status" id="maskingSoftware.status"></td> |
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<td class="help"> |
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<span id="maskingSoftware.message" class="message"></span> |
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Select the software to use for masking. |
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</td> |
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</tr> |
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</tbody> |
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<tbody class="info-section"> |
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<tr> |
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<td class="subprompt">Parameter set</td> |
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<td class="info" colspan="2"> |
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<span id="maskingSoftware.parameterSet"></span> |
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<div id="maskingSoftware.description" class="item-description"></div> |
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</td> |
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<td class="help"> |
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This step uses Bowtie2 for masking unwanted sequences. Parameters are configured in |
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<legacy> section in reggie-config.xml. |
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</td> |
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</tr> |
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</tbody> |
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<tbody> |
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<tr class="section-header"> |
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<td colspan="4">Align</td> |
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</tr> |
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<tr valign="top"> |
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<td class="subprompt">Protocol</td> |
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<td class="input"> |
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<select name="alignProtocol" id="alignProtocol"></select> |
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</td> |
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<td class="status" id="alignProtocol.status"></td> |
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<td class="help"> |
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<span id="alignProtocol.message" class="message"></span> |
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Select the protocol used for alignment. |
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</td> |
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</tr> |
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<tr> |
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<td class="subprompt">Software</td> |
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<td class="input"> |
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<select name="alignSoftware" id="alignSoftware"></select> |
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</td> |
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<td class="status" id="alignSoftware.status"></td> |
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<td class="help"> |
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<span id="alignSoftware.message" class="message"></span> |
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Select the software to use for alignment. |
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</td> |
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</tr> |
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</tbody> |
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<tbody class="info-section"> |
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<tr> |
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<td class="subprompt">Parameter set</td> |
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<td class="info" colspan="2"> |
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<span id="alignSoftware.parameterSet"></span> |
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<div id="alignSoftware.description" class="item-description"></div> |
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</td> |
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<td class="help"> |
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This step uses Tophat for for aligning sequences. After the alignment |
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it will run Picard MarkDuplicates and AddOrReplaceReadGroups as well |
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as a few other tools for statistics. Parameters are configured in |
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<legacy> section in reggie-config.xml. |
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</td> |
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</tr> |
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</tbody> |
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<tbody> |
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<tr class="section-header"> |
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<td colspan="4">Cufflinks</td> |
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</tr> |
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<tr> |
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<td class="prompt">Array design</td> |
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<td class="input"> |
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<select name="arrayDesign" id="arrayDesign" class="required"></select> |
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</td> |
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<td class="status" id="arrayDesign.status"></td> |
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<td class="help"> |
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<span id="arrayDesign.message" class="message"></span> |
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Select the array design to link the data to. |
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</td> |
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</tr> |
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<tr> |
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<td class="prompt">Protocol</td> |
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<td class="input"> |
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<select name="cufflinksProtocol" id="cufflinksProtocol"></select> |
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</td> |
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<td class="status" id="cufflinksProtocol.status"></td> |
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<td class="help"> |
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<span id="cufflinksProtocol.message" class="message"></span> |
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Select the protocol used. |
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</td> |
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</tr> |
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<tr> |
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<td class="prompt">Software</td> |
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<td class="input"> |
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<select name="cufflinksSoftware" id="cufflinksSoftware"></select> |
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</td> |
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<td class="status" id="cufflinksSoftware.status"></td> |
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<td class="help"> |
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<span id="cufflinksSoftware.message" class="message"></span> |
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Select the software to use. |
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</td> |
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</tr> |
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</tbody> |
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<tbody class="info-section"> |
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<tr> |
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<td class="subprompt">Parameter set</td> |
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<td class="info" colspan="2"> |
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<span id="cufflinksSoftware.parameterSet"></span> |
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<div id="cufflinksSoftware.description" class="item-description"></div> |
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</td> |
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<td class="help"> |
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This step uses Cufflinks for calculating expression values. |
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Parameters are configured in <legacy> section in reggie-config.xml. |
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</td> |
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</tr> |
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</tbody> |
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<tbody> |
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<tr class="section-header"> |
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<td colspan="4">Job scheduler</td> |
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</tr> |
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<tr> |
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<td class="subprompt">Cluster</td> |
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<td class="input"> |
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<select name="clusters" id="clusters" class="required"></select></td> |
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<td class="status" id="clusters.status"></td> |
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<td class="help"> |
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02 Sep 14 |
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<span id="clusters.message" class="message"></span> |
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02 Sep 14 |
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Select the cluster to use for running the software. |
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02 Sep 14 |
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</td> |
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02 Sep 14 |
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</tr> |
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02 Sep 14 |
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<tr id="job-priority" style="display: none;"> |
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02 Sep 14 |
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<td class="subprompt">Job priority</td> |
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02 Sep 14 |
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<td class="input"> |
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02 Sep 14 |
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<select name="priority" id="priority"></select> |
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</td> |
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<td class="status" id="priority.status"></td> |
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02 Sep 14 |
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<td class="help"> |
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02 Sep 14 |
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<span id="priority.message" class="message"></span> |
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02 Sep 14 |
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Select the priority of the job. |
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02 Sep 14 |
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</td> |
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02 Sep 14 |
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</tr> |
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<tr id="job-partition" style="display: none;"> |
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17 Jan 23 |
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<td class="subprompt">Job partition</td> |
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17 Jan 23 |
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<td class="input"> |
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17 Jan 23 |
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<select name="partition" id="partition"></select> |
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17 Jan 23 |
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</td> |
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<td class="status" id="partition.status"></td> |
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17 Jan 23 |
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<td class="help"> |
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17 Jan 23 |
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<span id="partition.message" class="message"></span> |
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17 Jan 23 |
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Select the partition/queue that the job should be submitted to. |
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17 Jan 23 |
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</td> |
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17 Jan 23 |
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</tr> |
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06 Oct 23 |
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</tbody> |
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06 Oct 23 |
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<tbody class="info-section"> |
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06 Oct 23 |
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<tr class="align-top"> |
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06 Oct 23 |
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<td class="subprompt">Job options</td> |
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06 Oct 23 |
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<td class="info input"> |
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06 Oct 23 |
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<div id="submitOptions" class="item-description code"></div> |
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06 Oct 23 |
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<textarea id="submitOptionsOverride" name="submitOptionsOverride" rows="4" style="display: none;"></textarea> |
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06 Oct 23 |
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</td> |
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06 Oct 23 |
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<td class="status" id="submitOptions.status"> |
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06 Oct 23 |
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<input type="checkbox" id="override" name="override" title="Override the default options" disabled> |
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06 Oct 23 |
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</td> |
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06 Oct 23 |
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<td class="help"> |
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06 Oct 23 |
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Parameters are configured in the <legacy>/<submit> |
7372 |
06 Oct 23 |
nicklas |
258 |
section in reggie-config.xml. Select the checkbox to manually |
7372 |
06 Oct 23 |
nicklas |
259 |
override the default options. |
7372 |
06 Oct 23 |
nicklas |
260 |
</td> |
7372 |
06 Oct 23 |
nicklas |
261 |
</tr> |
7372 |
06 Oct 23 |
nicklas |
262 |
</tbody> |
7372 |
06 Oct 23 |
nicklas |
263 |
<tbody> |
2623 |
02 Sep 14 |
nicklas |
264 |
<tr> |
3029 |
11 Dec 14 |
nicklas |
265 |
<td class="subprompt">Auto-confirm</td> |
3029 |
11 Dec 14 |
nicklas |
266 |
<td class="input"><input type="checkbox" name="autoConfirm" checked></td> |
3029 |
11 Dec 14 |
nicklas |
267 |
<td class="status" id="autoConfirm.status"></td> |
3029 |
11 Dec 14 |
nicklas |
268 |
<td class="help"> |
3029 |
11 Dec 14 |
nicklas |
269 |
<span id="autoConfirm.message" class="message"></span> |
3029 |
11 Dec 14 |
nicklas |
270 |
If the alignment is successful and aligned pairs >1M it is automatically confirmed |
4542 |
26 Jun 17 |
nicklas |
271 |
and reports are created. If aligned pairs is <5M the RNA is flagged. |
3029 |
11 Dec 14 |
nicklas |
272 |
</td> |
3029 |
11 Dec 14 |
nicklas |
273 |
</tr> |
3029 |
11 Dec 14 |
nicklas |
274 |
<tr> |
2623 |
02 Sep 14 |
nicklas |
275 |
<td class="subprompt">Debug</td> |
7372 |
06 Oct 23 |
nicklas |
276 |
<td class="input"><input type="checkbox" name="debug" id="debug"></td> |
2623 |
02 Sep 14 |
nicklas |
277 |
<td class="status" id="debug.status"></td> |
2623 |
02 Sep 14 |
nicklas |
278 |
<td class="help"> |
2623 |
02 Sep 14 |
nicklas |
279 |
<span id="debug.message" class="message"></span> |
2623 |
02 Sep 14 |
nicklas |
280 |
When running in debug mode only part of the sequenced data is used and |
3029 |
11 Dec 14 |
nicklas |
281 |
temporary files are not removed from the cluster node. Note! The auto-confirm |
3029 |
11 Dec 14 |
nicklas |
282 |
rule is relaxed to >200k reads. |
2623 |
02 Sep 14 |
nicklas |
283 |
</td> |
2623 |
02 Sep 14 |
nicklas |
284 |
</tr> |
2623 |
02 Sep 14 |
nicklas |
285 |
</tbody> |
2375 |
24 Apr 14 |
nicklas |
286 |
</table> |
2599 |
25 Aug 14 |
nicklas |
287 |
</div> |
2375 |
24 Apr 14 |
nicklas |
288 |
</div> |
2375 |
24 Apr 14 |
nicklas |
289 |
|
2599 |
25 Aug 14 |
nicklas |
290 |
<div id="wizard-status"></div> |
2375 |
24 Apr 14 |
nicklas |
291 |
|
2599 |
25 Aug 14 |
nicklas |
292 |
<table class="navigation" id="navigation"> |
2375 |
24 Apr 14 |
nicklas |
293 |
<tr> |
2599 |
25 Aug 14 |
nicklas |
294 |
<td><base:button id="gocancel" title="Cancel" /></td> |
2599 |
25 Aug 14 |
nicklas |
295 |
<td><base:button id="gonext" title="Next" image="<%=home+"/images/gonext.png"%>" /></td> |
2599 |
25 Aug 14 |
nicklas |
296 |
<td><base:button id="goregister" title="Register" image="<%=home+"/images/import.png"%>" /></td> |
2599 |
25 Aug 14 |
nicklas |
297 |
<td><base:button id="gorestart" title="Restart" image="<%=home+"/images/goback.png"%>" /></td> |
2602 |
27 Aug 14 |
nicklas |
298 |
<td id="gonext-message" class="message"></td> |
2375 |
24 Apr 14 |
nicklas |
299 |
</tr> |
2599 |
25 Aug 14 |
nicklas |
300 |
</table> |
2375 |
24 Apr 14 |
nicklas |
301 |
|
2375 |
24 Apr 14 |
nicklas |
302 |
</form> |
2375 |
24 Apr 14 |
nicklas |
303 |
</div> |
2375 |
24 Apr 14 |
nicklas |
304 |
|
2375 |
24 Apr 14 |
nicklas |
305 |
</base:body> |
2375 |
24 Apr 14 |
nicklas |
306 |
</base:page> |
2375 |
24 Apr 14 |
nicklas |
307 |
<% |
2375 |
24 Apr 14 |
nicklas |
308 |
} |
2375 |
24 Apr 14 |
nicklas |
309 |
finally |
2375 |
24 Apr 14 |
nicklas |
310 |
{ |
2375 |
24 Apr 14 |
nicklas |
311 |
if (dc != null) dc.close(); |
2375 |
24 Apr 14 |
nicklas |
312 |
} |
2375 |
24 Apr 14 |
nicklas |
313 |
%> |