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<%@ page |
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pageEncoding="UTF-8" |
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session="false" |
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import="net.sf.basedb.core.User" |
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import="net.sf.basedb.core.DbControl" |
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import="net.sf.basedb.core.SessionControl" |
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import="net.sf.basedb.core.Application" |
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import="net.sf.basedb.clients.web.Base" |
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import="net.sf.basedb.clients.web.util.HTML" |
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import="net.sf.basedb.clients.web.extensions.ExtensionsControl" |
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%> |
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<%@ taglib prefix="base" uri="/WEB-INF/base.tld" %> |
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<%@ taglib prefix="p" uri="/WEB-INF/path.tld" %> |
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<% |
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final SessionControl sc = Base.getExistingSessionControl(request, "net.sf.basedb.reggie", true); |
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final String ID = sc.getId(); |
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final float scale = Base.getScale(sc); |
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final String home = ExtensionsControl.getHomeUrl("net.sf.basedb.reggie"); |
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final String pipeline = request.getParameter("pipeline"); |
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DbControl dc = null; |
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try |
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{ |
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dc = sc.newDbControl(); |
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final User user = User.getById(dc, sc.getLoggedInUserId()); |
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%> |
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<base:page type="default" > |
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<base:head |
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scripts="~../reggie-2.js,~demux_start.js" |
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styles="path.css,~../css/reggie-2.css" |
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> |
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<style> |
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.notify.donotuseinfo |
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{ |
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width: auto; |
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max-width: 60em; |
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font-weight: normal; |
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background-color: #F8F8E8; |
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border-color: inherit; |
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} |
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|
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.notify.donotuseinfo > div:first-child > div |
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{ |
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border-color: #F8F8E8; |
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} |
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|
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.notify .notify-message:before |
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{ |
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content: url('../images/info.png'); |
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} |
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|
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.donotuseinfo td |
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{ |
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vertical-align: top; |
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padding: 2px; |
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} |
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</style> |
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|
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</base:head> |
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<base:body> |
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|
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<p:path><p:pathelement |
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title="Reggie" href="<%="../index.jsp?ID="+ID%>" |
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/><p:pathelement title="Start demux and merge" |
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/></p:path> |
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|
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<div id="page-data" class="datacontainer" |
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data-pipeline="<%=HTML.encodeTags(pipeline)%>" |
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></div> |
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|
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<div class="content"> |
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<form name="reggie" id="wizard" class="wizard"> |
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|
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<div class="step auto-hide" id="step-1"> |
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<div class="step-no">1</div> |
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<div class="step-title">Select sequencing runs</div> |
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<div class="step-content"> |
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|
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<table class="step-form"> |
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<tr class="align-top"> |
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<td class="prompt">Sequencing runs</td> |
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<td class="input"> |
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<select class="required" name="seqRuns" id="seqRuns" |
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multiple size="8"></select> |
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|
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<base:buttongroup style="margin-top: 0.5em;"> |
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<base:button title="Select manually…" id="btnSelectSeqRuns" /> |
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</base:buttongroup> |
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</td> |
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<td class="status" id="seqRuns.status"></td> |
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<td class="help"> |
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<span id="seqRuns.message" class="message"></span> |
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Select the sequencing runs that should be demuxed. Data for libraries that are found |
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in multiple places are merged to a single item for that library. |
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</td> |
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</tr> |
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</table> |
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</div> |
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</div> |
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|
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<div class="step" id="step-2"> |
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<div class="step-no">2</div> |
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<div class="step-title">Demux and merge information</div> |
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<div class="step-content"> |
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|
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<table class="step-form"> |
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<tbody> |
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<tr class="section-header"> |
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<td colspan="4">Demux</td> |
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</tr> |
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<tr> |
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<td class="subprompt">Protocol</td> |
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<td class="input"> |
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<select name="demuxProtocol" id="demuxProtocol"></select> |
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</td> |
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<td class="status" id="demuxProtocol.status"></td> |
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<td class="help"> |
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<span id="demuxProtocol.message" class="message"></span> |
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Select the protocol used for demuxing. |
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</td> |
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</tr> |
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<tr> |
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<td class="subprompt">Software</td> |
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<td class="input"> |
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<select name="demuxSoftware" id="demuxSoftware"></select> |
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</td> |
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<td class="status" id="demuxSoftware.status"></td> |
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<td class="help"> |
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<span id="demuxSoftware.message" class="message"></span> |
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Select the software to use for demuxing. |
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</td> |
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</tr> |
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</tbody> |
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<tbody class="info-section"> |
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<tr> |
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<td class="subprompt">Parameter set</td> |
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<td class="info" colspan="2"> |
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<span id="demuxSoftware.parameterSet"></span> |
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<div id="demuxSoftware.description" class="item-description"></div> |
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</td> |
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<td class="help"> |
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This step uses Picard to extract barcodes and extract sequence information to FASTQ files. |
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Parameters are configured in the <demux> section in reggie-config.xml but note that the |
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Bowtie and Trimmomatic parameters are for the merge step. |
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</td> |
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</tr> |
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</tbody> |
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<tbody id="SequencingRun-section"></tbody> |
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<tbody> |
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<tr> |
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<td class="prompt">Libraries to demux</td> |
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<td class="input"> |
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09 Jun 21 |
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<label><input type="radio" name="whichLibs" id="libsAll" value="all" checked>All</label><br> |
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<span id="doNotUseOption" style="display: none;"> |
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09 Jun 21 |
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<label><input type="radio" name="whichLibs" id="libsExceptDoNotUse" value="allExceptDoNotUse" |
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>All, except <span id="numDoNotUseLibs"></span> marked with DoNotUse</label> |
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<img src="../images/info.png" class="link" id="donotuseinfo" title="More information about the libraries..."><br> |
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</span> |
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09 Jun 21 |
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<label><input type="radio" name="whichLibs" id="libsSelected" value="selected">Select...</label> |
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<span id="libsSelectedLabel"></span> |
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</td> |
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<td class="status" id="whichLibs.status"></td> |
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<td class="help"> |
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<span id="whichLibs.message" class="message"></span> |
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09 Jun 21 |
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Select which libraries that should be included in the demux. |
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</td> |
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</tr> |
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</tbody> |
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<tbody> |
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<tr class="section-header"> |
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<td colspan="4">Merge</td> |
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02 Sep 14 |
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</tr> |
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<tr> |
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<td class="subprompt">Protocol</td> |
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<td class="input"> |
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<select name="mergeProtocol" id="mergeProtocol"></select> |
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</td> |
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<td class="status" id="mergeProtocol.status"></td> |
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<td class="help"> |
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<span id="mergeProtocol.message" class="message"></span> |
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10 Feb 15 |
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Select the protocol used for merging. |
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</td> |
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10 Feb 15 |
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</tr> |
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<tr> |
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<td class="subprompt">Software</td> |
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<td class="input"> |
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<select name="mergeSoftware" id="mergeSoftware"></select> |
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</td> |
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<td class="status" id="mergeSoftware.status"></td> |
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<td class="help"> |
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02 Sep 14 |
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<span id="mergeSoftware.message" class="message"></span> |
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02 Sep 14 |
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Select the software to use for merging the FASTQ files. |
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</td> |
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</tr> |
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</tbody> |
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<tbody class="info-section"> |
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<tr> |
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<td class="subprompt">Parameter set</td> |
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<td class="info" colspan="2"> |
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<span id="mergeSoftware.parameterSet"></span> |
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<div id="mergeSoftware.description" class="item-description"></div> |
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</td> |
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<td class="help"> |
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This step will merge the FASTQ files into two files per sample (R1+R2) and |
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then run Bowtie and Trimmomatic for some statistics and cleanup. |
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Parameters are configured in the |
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<demux> section in reggie-config.xml. |
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</td> |
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</tr> |
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</tbody> |
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<tbody> |
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<tr class="section-header"> |
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<td colspan="4">Job scheduler & job options</td> |
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</tr> |
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02 Sep 14 |
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<tr> |
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<td class="subprompt">Cluster</td> |
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<td class="input"> |
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<select name="clusters" id="clusters" class="required"></select> |
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</td> |
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<td class="status" id="clusters.status"></td> |
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<td class="help"> |
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02 Sep 14 |
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<span id="clusters.message" class="message"></span> |
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02 Sep 14 |
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Select the cluster to use for running the software. |
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02 Sep 14 |
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</td> |
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02 Sep 14 |
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</tr> |
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02 Sep 14 |
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<tr id="job-priority" style="display: none;"> |
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<td class="subprompt">Job priority</td> |
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02 Sep 14 |
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<td class="input"> |
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02 Sep 14 |
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<select name="priority" id="priority"></select> |
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02 Sep 14 |
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</td> |
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02 Sep 14 |
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<td class="status" id="priority.status"></td> |
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02 Sep 14 |
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<td class="help"> |
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02 Sep 14 |
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<span id="priority.message" class="message"></span> |
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02 Sep 14 |
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Select the priority of the job. |
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02 Sep 14 |
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</td> |
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02 Sep 14 |
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</tr> |
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<tr id="job-partition" style="display: none;"> |
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17 Jan 23 |
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<td class="subprompt">Job partition</td> |
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17 Jan 23 |
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<td class="input"> |
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17 Jan 23 |
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<select name="partition" id="partition"></select> |
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17 Jan 23 |
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</td> |
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17 Jan 23 |
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<td class="status" id="partition.status"></td> |
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17 Jan 23 |
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<td class="help"> |
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17 Jan 23 |
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<span id="partition.message" class="message"></span> |
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17 Jan 23 |
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Select the partition/queue that the job should be submitted to. |
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17 Jan 23 |
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</td> |
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17 Jan 23 |
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</tr> |
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06 Oct 23 |
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</tbody> |
7372 |
06 Oct 23 |
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<tbody class="info-section"> |
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06 Oct 23 |
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249 |
<tr class="align-top"> |
7372 |
06 Oct 23 |
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250 |
<td class="subprompt">Job options</td> |
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06 Oct 23 |
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251 |
<td class="info input"> |
7372 |
06 Oct 23 |
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252 |
<div id="submitOptions" class="item-description code"></div> |
7372 |
06 Oct 23 |
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253 |
<textarea id="submitOptionsOverride" name="submitOptionsOverride" rows="4" style="display: none;"></textarea> |
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19 Nov 14 |
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254 |
</td> |
7372 |
06 Oct 23 |
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255 |
<td class="status" id="submitOptions.status"> |
7372 |
06 Oct 23 |
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256 |
<input type="checkbox" id="override" name="override" title="Override the default options" disabled> |
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06 Oct 23 |
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257 |
</td> |
2960 |
19 Nov 14 |
nicklas |
258 |
<td class="help"> |
7372 |
06 Oct 23 |
nicklas |
259 |
Parameters are configured in the <demux>/<submit> |
7372 |
06 Oct 23 |
nicklas |
260 |
section in reggie-config.xml. Select the checkbox to manually |
7372 |
06 Oct 23 |
nicklas |
261 |
override the default options. |
2960 |
19 Nov 14 |
nicklas |
262 |
</td> |
2960 |
19 Nov 14 |
nicklas |
263 |
</tr> |
7372 |
06 Oct 23 |
nicklas |
264 |
</tbody> |
7372 |
06 Oct 23 |
nicklas |
265 |
<tbody> |
2960 |
19 Nov 14 |
nicklas |
266 |
<tr> |
3029 |
11 Dec 14 |
nicklas |
267 |
<td class="subprompt">Auto-confirm</td> |
3029 |
11 Dec 14 |
nicklas |
268 |
<td class="input"><input type="checkbox" name="autoConfirm" checked></td> |
3029 |
11 Dec 14 |
nicklas |
269 |
<td class="status" id="autoConfirm.status"></td> |
3029 |
11 Dec 14 |
nicklas |
270 |
<td class="help"> |
3029 |
11 Dec 14 |
nicklas |
271 |
<span id="autoConfirm.message" class="message"></span> |
3029 |
11 Dec 14 |
nicklas |
272 |
If the demux is successful, without warnings and all libraries get >10M reads after trimmomatic |
7372 |
06 Oct 23 |
nicklas |
273 |
and valid fragment size values, it is automatically confirmed and alignment is started. |
3029 |
11 Dec 14 |
nicklas |
274 |
</td> |
3029 |
11 Dec 14 |
nicklas |
275 |
</tr> |
3029 |
11 Dec 14 |
nicklas |
276 |
<tr> |
2622 |
02 Sep 14 |
nicklas |
277 |
<td class="subprompt">Debug</td> |
7372 |
06 Oct 23 |
nicklas |
278 |
<td class="input"><input type="checkbox" name="debug" id="debug"></td> |
2622 |
02 Sep 14 |
nicklas |
279 |
<td class="status" id="debug.status"></td> |
2622 |
02 Sep 14 |
nicklas |
280 |
<td class="help"> |
2622 |
02 Sep 14 |
nicklas |
281 |
<span id="debug.message" class="message"></span> |
2622 |
02 Sep 14 |
nicklas |
282 |
When running in debug mode only part of the sequenced data is used and |
3029 |
11 Dec 14 |
nicklas |
283 |
temporary files are not removed from the cluster node. Note! The auto-confirm |
3029 |
11 Dec 14 |
nicklas |
284 |
rule is relaxed to >300k reads. |
2622 |
02 Sep 14 |
nicklas |
285 |
</td> |
2622 |
02 Sep 14 |
nicklas |
286 |
</tr> |
2622 |
02 Sep 14 |
nicklas |
287 |
</tbody> |
2268 |
05 Mar 14 |
nicklas |
288 |
</table> |
2622 |
02 Sep 14 |
nicklas |
289 |
</div> |
2268 |
05 Mar 14 |
nicklas |
290 |
</div> |
2268 |
05 Mar 14 |
nicklas |
291 |
|
2622 |
02 Sep 14 |
nicklas |
292 |
<div id="wizard-status"></div> |
2268 |
05 Mar 14 |
nicklas |
293 |
|
2622 |
02 Sep 14 |
nicklas |
294 |
<table class="navigation" id="navigation"> |
2268 |
05 Mar 14 |
nicklas |
295 |
<tr> |
2622 |
02 Sep 14 |
nicklas |
296 |
<td><base:button id="gocancel" title="Cancel" /></td> |
2622 |
02 Sep 14 |
nicklas |
297 |
<td><base:button id="gonext" title="Next" image="<%=home+"/images/gonext.png"%>" /></td> |
2622 |
02 Sep 14 |
nicklas |
298 |
<td><base:button id="goregister" title="Register" image="<%=home+"/images/import.png"%>" /></td> |
2622 |
02 Sep 14 |
nicklas |
299 |
<td><base:button id="gorestart" title="Restart" image="<%=home+"/images/goback.png"%>" /></td> |
2622 |
02 Sep 14 |
nicklas |
300 |
<td id="gonext-message" class="message"></td> |
2268 |
05 Mar 14 |
nicklas |
301 |
</tr> |
2622 |
02 Sep 14 |
nicklas |
302 |
</table> |
2268 |
05 Mar 14 |
nicklas |
303 |
|
2268 |
05 Mar 14 |
nicklas |
304 |
</form> |
2268 |
05 Mar 14 |
nicklas |
305 |
</div> |
2268 |
05 Mar 14 |
nicklas |
306 |
|
2268 |
05 Mar 14 |
nicklas |
307 |
</base:body> |
2268 |
05 Mar 14 |
nicklas |
308 |
</base:page> |
2268 |
05 Mar 14 |
nicklas |
309 |
<% |
2268 |
05 Mar 14 |
nicklas |
310 |
} |
2268 |
05 Mar 14 |
nicklas |
311 |
finally |
2268 |
05 Mar 14 |
nicklas |
312 |
{ |
2268 |
05 Mar 14 |
nicklas |
313 |
if (dc != null) dc.close(); |
2268 |
05 Mar 14 |
nicklas |
314 |
} |
2268 |
05 Mar 14 |
nicklas |
315 |
%> |