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package net.sf.basedb.reggie.dao; |
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import java.util.ArrayList; |
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import java.util.Collection; |
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import java.util.List; |
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|
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import org.json.simple.JSONObject; |
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|
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import net.sf.basedb.core.DbControl; |
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import net.sf.basedb.core.DerivedBioAssay; |
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import net.sf.basedb.core.Extract; |
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import net.sf.basedb.core.Include; |
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import net.sf.basedb.core.InvalidDataException; |
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import net.sf.basedb.core.ItemQuery; |
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import net.sf.basedb.core.Job; |
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import net.sf.basedb.core.Type; |
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import net.sf.basedb.core.query.Expressions; |
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import net.sf.basedb.core.query.Hql; |
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import net.sf.basedb.core.query.Orders; |
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import net.sf.basedb.core.query.Restrictions; |
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import net.sf.basedb.reggie.Reggie; |
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|
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/** |
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Class for loading information that is related to a sequencing run. |
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|
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@author nicklas |
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@since 2.13 |
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*/ |
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public class SequencingRun |
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extends ReggieItem<DerivedBioAssay> |
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{ |
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|
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/** |
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Value for the {@link Annotationtype#SEQUENCING_RESULT} annotation |
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when the sequencing was successful. |
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@since 2.15 |
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*/ |
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public static final String SEQUENCING_SUCCESSFUL = "Successful"; |
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|
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/** |
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Value for the {@link Annotationtype#SEQUENCING_RESULT} annotation when |
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the sequencing failed and a new flow cell must be used for re-sequencing. |
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@since 2.15 |
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*/ |
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public static final String SEQUENCING_FAILED = "Failed"; |
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|
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/** |
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Value for the {@link Annotationtype#SEQUENCING_RESULT} annotation when |
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the first base report failed and the same flow cell can be used |
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for re-sequencing. |
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@since 2.15 |
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*/ |
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public static final String SEQUENCING_FIRST_BASE_REPORT_FAILED = "FirstBaseReportFailed"; |
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|
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/** |
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Get a sequencing run when the id is known. |
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*/ |
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public static SequencingRun getById(DbControl dc, int id) |
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{ |
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return new SequencingRun(DerivedBioAssay.getById(dc, id)); |
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} |
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|
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public static SequencingRun get(DerivedBioAssay dba) |
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{ |
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return new SequencingRun(dba); |
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} |
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|
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/** |
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Load the sequencing run represented by the given job. |
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@since 4.1 |
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*/ |
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public static SequencingRun getByJob(DbControl dc, Job job) |
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{ |
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ItemQuery<DerivedBioAssay> query = DerivedBioAssay.getQuery(); |
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query.setIncludes(Reggie.INCLUDE_IN_CURRENT_PROJECT); |
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Subtype.SEQUENCING_RUN.addFilter(dc, query); |
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query.restrict(Restrictions.eq(Hql.property("job"), Hql.entity(job))); |
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|
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List<DerivedBioAssay> list = query.list(dc); |
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if (list.size() > 1) |
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{ |
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throw new InvalidDataException( |
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"More than one item with job " + job.getName() + " was found. " + |
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"This wizard can't be used until that is corrected."); |
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} |
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SequencingRun seqRun = null; |
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if (list.size() == 1) |
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{ |
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seqRun = new SequencingRun(list.get(0)); |
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} |
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return seqRun; |
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} |
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|
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/** |
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Load the sequencing run for a demuxed sequences item. |
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@since 2.16 |
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*/ |
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public static SequencingRun getByDemuxedSequences(DbControl dc, DemuxedSequences demux) |
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{ |
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ItemQuery<DerivedBioAssay> query = DerivedBioAssay.getQuery(); |
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Subtype.SEQUENCING_RUN.addFilter(dc, query); |
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query.include(Reggie.INCLUDE_IN_CURRENT_PROJECT); |
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|
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// Join demuxed sequences runs |
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query.join(Hql.innerJoin("children", "dx")); |
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query.restrict(Restrictions.eq(Hql.alias("dx"), Hql.entity(demux.getItem()))); |
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|
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query.order(Orders.asc(Hql.property("name"))); |
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|
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List<DerivedBioAssay> tmp = query.list(dc); |
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DerivedBioAssay seqRun = null; |
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if (tmp.size() > 1) |
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{ |
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throw new InvalidDataException( |
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"More than one sequencing run was found for " + demux.getName() + |
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"This wizard can't be used until that is corrected."); |
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} |
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else if (tmp.size() == 1) |
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{ |
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seqRun = tmp.get(0); |
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} |
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return seqRun == null ? null : new SequencingRun(seqRun); |
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} |
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|
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|
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/** |
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Find all sequencing runs that include the library with the given case name. |
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This method will check for {@link Subtype#SEQUENCING_RUN} derived bioassays |
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that have a parent flow cell that include pools that contains libraries |
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with the given name. |
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@since 2.15 |
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*/ |
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public static List<SequencingRun> findByCaseName(DbControl dc, String name) |
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{ |
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// Get rid of suffixes in the name (eg. 'C' which is used for pre-neoadjuvant forms) |
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if (name.length() > 7) name = name.substring(0, 7); |
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|
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// Find all 'Pooled library aliquot':s where the lib we are looking for is present |
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ItemQuery<Extract> poolQuery = Extract.getQuery(); |
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Subtype.POOLED_LIBRARY_ALIQUOT.addFilter(dc, poolQuery); |
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poolQuery.setIncludes(Reggie.INCLUDE_IN_CURRENT_PROJECT); |
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poolQuery.join(Hql.innerJoin("parent", "pool")); |
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poolQuery.join(Hql.innerJoin("pool", "creationEvent", "poolc")); |
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poolQuery.join(Hql.innerJoin("poolc", "sources", "poolsrc")); |
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poolQuery.join(Hql.innerJoin("poolsrc", "bioMaterial", "lib")); |
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poolQuery.restrict(Restrictions.like(Hql.property("lib", "name"), Expressions.parameter("name", name+".%", Type.STRING))); |
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|
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// Find all Sequencing run items for flow cells that include any of the pools. |
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ItemQuery<DerivedBioAssay> query = DerivedBioAssay.getQuery(); |
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Subtype.SEQUENCING_RUN.addFilter(dc, query); |
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query.setIncludes(Reggie.INCLUDE_IN_CURRENT_PROJECT); |
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query.join(Hql.innerJoin(null, "physicalBioAssays", "fc", true)); |
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query.join(Hql.innerJoin("fc", "creationEvent", "fcc", true)); |
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query.join(Hql.innerJoin("fcc", "sources", "src")); |
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query.join(Hql.innerJoin("src", "bioMaterial", "poolA")); |
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query.restrict(Restrictions.eq(Hql.alias("poolA"), Expressions.any(poolQuery))); |
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query.order(Orders.asc(Hql.property("name"))); |
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|
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return toList(query.list(dc)); |
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} |
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|
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/** |
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Find all sequencing runs that include the given library. |
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This method will check for {@link Subtype#SEQUENCING_RUN} derived bioassays |
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that have a parent flow cell that include pools that contains the given library. |
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@since 4.7 |
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*/ |
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public static List<SequencingRun> findByLibrary(DbControl dc, Library lib) |
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{ |
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// Find all 'Pooled library aliquot':s where the lib we are looking for is present |
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ItemQuery<Extract> poolQuery = Extract.getQuery(); |
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Subtype.POOLED_LIBRARY_ALIQUOT.addFilter(dc, poolQuery); |
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poolQuery.setIncludes(Reggie.INCLUDE_IN_CURRENT_PROJECT); |
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poolQuery.join(Hql.innerJoin("parent", "pool")); |
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poolQuery.join(Hql.innerJoin("pool", "creationEvent", "poolc")); |
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poolQuery.join(Hql.innerJoin("poolc", "sources", "poolsrc")); |
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poolQuery.join(Hql.innerJoin("poolsrc", "bioMaterial", "lib")); |
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poolQuery.restrict(Restrictions.eq(Hql.alias("lib"), Hql.entity(lib.getExtract()))); |
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|
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// Find all Sequencing run items for flow cells that include any of the pools. |
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ItemQuery<DerivedBioAssay> query = DerivedBioAssay.getQuery(); |
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Subtype.SEQUENCING_RUN.addFilter(dc, query); |
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query.setIncludes(Reggie.INCLUDE_IN_CURRENT_PROJECT); |
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query.join(Hql.innerJoin(null, "physicalBioAssays", "fc", true)); |
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query.join(Hql.innerJoin("fc", "creationEvent", "fcc", true)); |
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query.join(Hql.innerJoin("fcc", "sources", "src")); |
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query.join(Hql.innerJoin("src", "bioMaterial", "poolA")); |
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query.restrict(Restrictions.eq(Hql.alias("poolA"), Expressions.any(poolQuery))); |
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query.order(Orders.asc(Hql.property("name"))); |
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|
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return toList(query.list(dc)); |
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} |
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|
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public static SequencingRun findByFlowCell(DbControl dc, FlowCell fc) |
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{ |
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ItemQuery<DerivedBioAssay> query = fc.getItem().getRootDerivedBioAssays(); |
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Subtype.SEQUENCING_RUN.addFilter(dc, query); |
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query.setIncludes(Reggie.INCLUDE_IN_CURRENT_PROJECT); |
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query.order(Orders.asc(Hql.property("name"))); |
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|
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List<DerivedBioAssay> tmp = query.list(dc); |
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|
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DerivedBioAssay seqRun = null; |
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if (tmp.size() > 1) |
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{ |
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throw new InvalidDataException( |
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"More than one sequencing run was found for " + fc.getName() + |
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"This wizard can't be used until that is corrected."); |
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} |
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else if (tmp.size() == 1) |
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{ |
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seqRun = tmp.get(0); |
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} |
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|
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return seqRun == null ? null : new SequencingRun(seqRun); |
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} |
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|
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public static List<SequencingRun> toList(Collection<DerivedBioAssay> bioassays) |
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{ |
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List<SequencingRun> lib = new ArrayList<SequencingRun>(bioassays.size()); |
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for (DerivedBioAssay ba : bioassays) |
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{ |
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lib.add(new SequencingRun(ba)); |
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} |
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return lib; |
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} |
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|
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|
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/** |
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04 Jul 18 |
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Generate a name for the new sequencing run. This method will search all |
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sequencing runs and find the one with the highest numeric suffix which |
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is incremented to create the next name. |
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*/ |
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public static String getNextName(DbControl dc) |
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{ |
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String prefix = Subtype.SEQUENCING_RUN.getItemPrefix(); |
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int numDigitsInName = 4; |
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ItemQuery<DerivedBioAssay> query = DerivedBioAssay.getQuery(); |
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Subtype.SEQUENCING_RUN.addFilter(dc, query); |
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query.include(Include.ALL); |
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return ReggieItem.getNextItemName(dc, query, prefix, numDigitsInName, true); |
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} |
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|
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|
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private SequencingRun(DerivedBioAssay bioAssay) |
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{ |
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super(bioAssay); |
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|
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} |
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|
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/** |
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07 Oct 13 |
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Get the derived bioassay that represents this sequencing run in BASE. |
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*/ |
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public DerivedBioAssay getDerivedBioAssay() |
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07 Oct 13 |
nicklas |
256 |
{ |
2049 |
07 Oct 13 |
nicklas |
257 |
return getItem(); |
2049 |
07 Oct 13 |
nicklas |
258 |
} |
2049 |
07 Oct 13 |
nicklas |
259 |
|
2049 |
07 Oct 13 |
nicklas |
260 |
@Override |
2049 |
07 Oct 13 |
nicklas |
261 |
protected void initJSON(JSONObject json) |
2049 |
07 Oct 13 |
nicklas |
262 |
{ |
2049 |
07 Oct 13 |
nicklas |
263 |
super.initJSON(json); |
2049 |
07 Oct 13 |
nicklas |
264 |
} |
2267 |
05 Mar 14 |
nicklas |
265 |
|
2267 |
05 Mar 14 |
nicklas |
266 |
/** |
2267 |
05 Mar 14 |
nicklas |
Find the next name to give a "DemuxedSequences" child item. This assumes that |
2376 |
24 Apr 14 |
nicklas |
all child items are using the naming convention. foo.x, foo.x2, and |
2267 |
05 Mar 14 |
nicklas |
so on. NOTE! The first child item have no number! |
2267 |
05 Mar 14 |
nicklas |
@return The next unused name |
2267 |
05 Mar 14 |
nicklas |
271 |
*/ |
2267 |
05 Mar 14 |
nicklas |
272 |
public String getNextDemuxedSequencesName(DbControl dc) |
2267 |
05 Mar 14 |
nicklas |
273 |
{ |
2267 |
05 Mar 14 |
nicklas |
274 |
DerivedBioAssay seqRun = getItem(); |
6193 |
30 Mar 21 |
nicklas |
275 |
ItemQuery<DerivedBioAssay> query = null; |
6193 |
30 Mar 21 |
nicklas |
276 |
if (seqRun.isInDatabase()) |
6193 |
30 Mar 21 |
nicklas |
277 |
{ |
6193 |
30 Mar 21 |
nicklas |
278 |
query = seqRun.getChildren(); |
6193 |
30 Mar 21 |
nicklas |
279 |
Subtype.DEMUXED_SEQUENCES.addFilter(dc, query); |
6193 |
30 Mar 21 |
nicklas |
280 |
query.setIncludes(Include.ALL); |
6193 |
30 Mar 21 |
nicklas |
281 |
} |
4882 |
03 Jul 18 |
nicklas |
282 |
return getNextChildItemName(dc, query, Subtype.DEMUXED_SEQUENCES.getItemSuffix(), true); |
2267 |
05 Mar 14 |
nicklas |
283 |
} |
2049 |
07 Oct 13 |
nicklas |
284 |
|
2049 |
07 Oct 13 |
nicklas |
285 |
} |