143 |
10 Aug 06 |
enell |
singleton - A spot occuring once |
143 |
10 Aug 06 |
enell |
duplicate - A spot occuring twice |
143 |
10 Aug 06 |
enell |
triplicate - ... |
143 |
10 Aug 06 |
enell |
replicate - A spot occuring more than once |
143 |
10 Aug 06 |
enell |
5 |
|
143 |
10 Aug 06 |
enell |
The concept of replicate error. |
143 |
10 Aug 06 |
enell |
7 |
|
143 |
10 Aug 06 |
enell |
The idea is that if you hav an experiemnt design that involve |
143 |
10 Aug 06 |
enell |
9 |
|
143 |
10 Aug 06 |
enell |
10 |
|
143 |
10 Aug 06 |
enell |
A spot must have a ratio value so all spots with one intensoty <= 0 are removed. |
143 |
10 Aug 06 |
enell |
12 |
|
143 |
10 Aug 06 |
enell |
* Replicates are created |
143 |
10 Aug 06 |
enell |
14 |
|
143 |
10 Aug 06 |
enell |
For mehtod "within assay" this means all spots in an assay whith the same |
143 |
10 Aug 06 |
enell |
reporter is a replicate. |
143 |
10 Aug 06 |
enell |
17 |
|
143 |
10 Aug 06 |
enell |
For method "across assay" this depends on how the assays are grouped |
143 |
10 Aug 06 |
enell |
according to the grouping parameters. This will work on spots "merged on |
143 |
10 Aug 06 |
enell |
reporter" |
143 |
10 Aug 06 |
enell |
21 |
|
143 |
10 Aug 06 |
enell |
* How replicates are used in the calculations |
143 |
10 Aug 06 |
enell |
23 |
|
143 |
10 Aug 06 |
enell |
The method is designed to |