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$Id$ |
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== Introduction == |
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This file gives some details on some of the plug-ins in this package. |
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== Background correction plug-in == |
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This plug-in will remove a per-slide global background from all |
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spots. The background is calculated from a set of negative control |
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spot on the array. See implementation details below for details. |
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=== Parameters === |
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There is one parameter to set that specifies how background |
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intensities should be calculated. Allowed values are median or mean, |
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i.e. the background is either the median or the mean of the negative |
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control spots on the array. |
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The expression of the background probes is optionally saved to a |
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file. The default is not to save the expression matrix but this can be |
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changed during job configuration. |
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=== Implementation details === |
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Each assay is treated separately, i.e., no samples are combined |
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together. All calculations are made on the current bioassay data |
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implying that this plug-in should be used early in analysis and before |
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background spots are removed. |
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A spot is considered to be a negative control spot if it has an |
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''External Id'' exactly matching the string ''(-)3xSLv1''. |
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---------------------------------------------------------------------- |
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{{{ |
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Copyright (C) 2010 Jari Häkkinen |
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This file is part of Illumina plug-in package for BASE. |
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Available at http://baseplugins.thep.lu.se/ |
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BASE main site: http://base.thep.lu.se/ |
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This is free software; you can redistribute it and/or modify it under |
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the terms of the GNU General Public License as published by the Free |
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Software Foundation; either version 3 of the License, or (at your |
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option) any later version. |
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The software is distributed in the hope that it will be useful, but |
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WITHOUT ANY WARRANTY; without even the implied warranty of |
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MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU |
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General Public License for more details. |
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You should have received a copy of the GNU General Public License |
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along with this program. If not, see <http://www.gnu.org/licenses/>. |
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}}} |
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---------------------------------------------------------------------- |