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<?xml version="1.0" encoding="UTF-8" ?> |
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<!-- |
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$Id$ |
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|
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Copyright (C) 2009 Martin Svensson |
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|
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This file is part of FTP extension for BASE. |
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Available at http://baseplugins.thep.lu.se/ |
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9 |
|
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BASE is free software; you can redistribute it and/or |
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modify it under the terms of the GNU General Public License |
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as published by the Free Software Foundation; either version 3 |
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of the License, or (at your option) any later version. |
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14 |
|
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BASE is distributed in the hope that it will be useful, |
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but WITHOUT ANY WARRANTY; without even the implied warranty of |
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MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the |
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GNU General Public License for more details. |
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19 |
|
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You should have received a copy of the GNU General Public License |
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along with this program; if not, write to the Free Software |
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Foundation, Inc., 59 Temple Place - Suite 330, |
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Boston, MA 02111-1307, USA. |
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--> |
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|
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<extensions xmlns="http://base.thep.lu.se/extensions.xsd"> |
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<about> |
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<name>Illumina extensions</name> |
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<version>1.8-dev</version> |
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<copyright>BASE development team</copyright> |
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<email>basedb-users@lists.sourceforge.net</email> |
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<url>http://baseplugins.thep.lu.se/wiki/net.sf.basedb.illumina</url> |
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<description> |
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This package contains plug-ins and extensions for the Illumina platform. |
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</description> |
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<min-base-version>3.3.0</min-base-version> |
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</about> |
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<plugin-definition id="Installer"> |
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<about> |
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<name>Illumina plug-in package installer</name> |
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<description> |
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This plug-in will install required platforms, data files types, etc. that |
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are required to use the Illumina plug-ins. Start the installation by selecting this |
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plug-in from the list at Administrate -> Plugins -> Definitions. Then click on the |
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'Run plugin' button. |
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</description> |
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</about> |
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<plugin-class>net.sf.basedb.illumina.install.Install</plugin-class> |
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</plugin-definition> |
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<plugin-definition id="ExpressionBackgroundCorrection"> |
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<about> |
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<name>Illumina expression background correction</name> |
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<description> |
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This plug-in will remove a per-slide global background from all |
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spots. The background is calculated from a set of negative control |
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spots on the array. |
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Each assay is treated separately, i.e., no samples are combined |
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together. All calculations are made on the current bioassay data |
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implying that this plug-in should be used early in analysis and before |
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background spots are removed. |
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A spot is considered to be a negative control spot if it has an |
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''Control group name'' exactly matching the string ''negative''. |
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There is one parameter to set that specifies how background |
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intensities should be calculated. Allowed values are median or mean, |
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i.e. the background is either the median or the mean of the negative |
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control spots on the array. |
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The expression of the background probes is optionally saved to a |
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file. The default is not to save the expression matrix but this can be |
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changed during job configuration. |
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</description> |
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</about> |
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<plugin-class>net.sf.basedb.illumina.plugins.BackgroundCorrection</plugin-class> |
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</plugin-definition> |
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<plugin-definition id="BeadSummaryImporter"> |
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<about> |
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<name>Illumina Bead Summary importer</name> |
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<description> |
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Raw data importer for Illumina bead summary files. This plug-in can import data from one |
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or more related bead summary files that are part of the same array but different stripes. |
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</description> |
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</about> |
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<plugin-class>net.sf.basedb.illumina.plugins.BeadSummaryImporter</plugin-class> |
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</plugin-definition> |
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<plugin-definition id="BgxFeatureImporter"> |
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<about> |
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<name>Illumina BGX feature importer</name> |
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<description> |
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Plugin that imports features from a Illumina BGX-file. |
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Both compressed(gzip) and uncompressed files are supported. |
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None of the mappings for the [Controls] section are configurable. |
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These are set like follows: |
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Identify features by->FEATURE_IDReporter ID->\\Probe_Id\\Feature ID->\\Array_Address_Id\\ |
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Reg exp for data header is preconfigured to 'Probe_Id\\tArray_Address_Id.*' |
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</description> |
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</about> |
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<plugin-class>net.sf.basedb.illumina.plugins.BgxFeatureImporter</plugin-class> |
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</plugin-definition> |
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<plugin-definition id="BgxReporterImporter"> |
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<about> |
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<name>Illumina BGX reporter importer</name> |
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<description> |
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Imports reporter annotation from Illumina BGX files. This importer is |
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based on the regular reporter importer and has the same features and functions. |
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It supports both compressed and uncompressed BGX files. File format configurations |
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made for this plug-in applies to the [Probes] section only. Column mappings for the |
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[Controls] section can't be configured. Some controls appear more than one time |
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in the [Controls] section. If the they have different values for the any of the |
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Reporter_Group_Name, Reporter_Group_Id and/or Reporter_Composite_Map columns the values |
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are merged with a ; as separator. |
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</description> |
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</about> |
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<plugin-class>net.sf.basedb.illumina.plugins.BgxReporterImporter</plugin-class> |
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</plugin-definition> |
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<plugin-definition id="DetectionPvalueCalculation"> |
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<about> |
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<name>Illumina detection P-value calculation</name> |
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<description> |
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This plug-in implements BeadStudio like detection P-value calculations |
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for Illumina expression data. |
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The plug-in will _always_ base the detection P-value calculation on |
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raw data values, i.e., the mean raw intensity for the different |
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signals. By default the calculations are based on negative controls |
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available in the root bioassay set for the current analysis |
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branch. The user may change this to only use negative controls in the |
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current bioassay set. |
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A data value is considered to be a negative control value if |
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it has a "Control group name" exactly matching the string |
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"negative". |
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The detection P-value plug-in does not filter the assays, it provides |
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the detection P-values usable in a filter step after running this |
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plug-in. |
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Parameters |
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- The plug-in requires input of array type since detection P-value are |
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calculated differently depending on array type. |
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- Users may select to use negative controls in the current bioassay set |
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only. The default behaviour is to use all negative controls in the |
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root bioassay set for the current bioassay set. |
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- A cut off parameter is available to exclude outliers within |
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the negative controls. The `cutoff` defines the acceptable negative |
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control signal range |
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$median-MAD*cutoff < I < median+MAD*cutoff$ |
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where $MAD$ is the median absolute deviation. |
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Implementation details |
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Each assay is treated separately, i.e., no samples are combined |
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together. All calculations are made on the raw bead-type level data, |
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i.e., on the average expression value for each bead type and raw data |
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is always used irrespective when in analysis the detection P-value is |
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calculated. |
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Pvalue calculation for whole genome arrays: |
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For all signals $i$ calculate the detection P-value as |
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$Pvalue = 1-R/N$ where $R$ is the rank of the signal $i$ relative to the |
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negative controls and $N$ is the number of negative controls. |
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Pvalue calculation for others array types (DASL, miRNA, VeraCode DASL, |
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and Focused Arrays): |
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For all signals $i$ calculate the detection P-value as |
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$Pvalue = 1/2 - 1/2 * erf( [i-AvgControl]/StdControl/sqrt(2) )$ where |
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$AvgControl$ is the average intensity of the negative controls, |
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$StdControl$ is the standard deviation of the the negative controls, |
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and $erf$ is the error function |
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(http://mathworld.wolfram.com/Erf.html). The error function is used |
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for arguments within the range (-4,4). To save CPU cycles, the function |
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value for arguments outside this range is set to -1 and 1, |
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respectively. |
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More details about this plug-in is found at the URL |
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Please send feedback to the email-address |
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</description> |
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</about> |
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<plugin-class>net.sf.basedb.illumina.plugins.DetectionPValue</plugin-class> |
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</plugin-definition> |
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<plugin-definition id="SnpRootBioAssaysetCreator"> |
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<about> |
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<name>Illumina SNP root bioassayset creator</name> |
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<description> |
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Creates a root bioassay set from Illumina SNP data. This plug-in works |
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only if all raw bioassays has the same array design and SNP split data files |
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are present. The root bioassayset is created by copying values from the data files |
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as follows: |
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* Channel 1 = GType (AA = 1.0, AB = 0.0, BB = -1.0, NC = null) |
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* Channel 2 = Log R Ratio |
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* Channel 3 = B Allele Freq |
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</description> |
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</about> |
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<plugin-class>net.sf.basedb.illumina.plugins.SnpIntensityCalculator</plugin-class> |
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</plugin-definition> |
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<plugin-definition id="SnpRawDataImporter"> |
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<about> |
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<name>Illumina SNP raw data importer</name> |
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<description> |
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This plug-in is used to import raw data for Illumina SNP from a tab separated text file. The plug-in can be |
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executed both from the list of raw bioassays and from within an experiment. In the |
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last case the imported data will be associated with the experiment |
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</description> |
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</about> |
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<plugin-class>net.sf.basedb.illumina.plugins.SnpRawDataImporter</plugin-class> |
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</plugin-definition> |
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<plugin-definition id="SnpReporterImporter"> |
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<about> |
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<name>Illumina SNP reporter importer</name> |
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<description> |
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Imports reporters from SNP manifest files. This importer works exactly |
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as the regular reporter importer and has the same features. |
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</description> |
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</about> |
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<plugin-class>net.sf.basedb.illumina.plugins.SnpReporterImporter</plugin-class> |
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</plugin-definition> |
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<extension |
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id="net.sf.basedb.illumina.extensions.overviewplots.illuminaPlots" |
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extends="net.sf.basedb.clients.web.bioassayset.overviewplots" |
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> |
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<index>20</index> |
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<about> |
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<name>Illumina overview plot</name> |
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23 Jun 09 |
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<description>Generates overview plot for Illumina expression data.</description> |
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</about> |
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<action-factory> |
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<factory-class>net.sf.basedb.illumina.extensions.plot.PlotFactory</factory-class> |
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<parameters> |
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<width>800</width> |
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<height>600</height> |
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</parameters> |
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</action-factory> |
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</extension> |
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<extension |
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id="net.sf.basedb.illumina.extensions.bgxvalidator" |
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06 Sep 11 |
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extends="net.sf.basedb.core.filehandler.validator" |
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> |
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<about> |
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<name>BGX file validator</name> |
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06 Sep 11 |
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<description> |
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06 Sep 11 |
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Validator and metadata extractor implementation for Illumina BGX files. |
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It will parse the start of the file until the [Probes] section is found |
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06 Sep 11 |
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and then extract the number of probes + controls and set that as the |
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number of features on the array design. |
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</description> |
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06 Sep 11 |
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</about> |
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06 Sep 11 |
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<index>1</index> |
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<action-factory> |
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06 Sep 11 |
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<factory-class>net.sf.basedb.illumina.filehandler.BgxValidationFactory</factory-class> |
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</action-factory> |
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06 Sep 11 |
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</extension> |
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06 Sep 11 |
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<extension |
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id="net.sf.basedb.illumina.extensions.snpvalidator" |
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06 Sep 11 |
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extends="net.sf.basedb.core.filehandler.validator" |
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06 Sep 11 |
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> |
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06 Sep 11 |
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<about> |
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06 Sep 11 |
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246 |
<name>SNP manifest validator</name> |
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06 Sep 11 |
nicklas |
247 |
<description> |
1386 |
06 Sep 11 |
nicklas |
248 |
Validator and metadata extractor implementation for Illumina SNP manifest files. |
1386 |
06 Sep 11 |
nicklas |
249 |
It will parse the start of the file until the [Assay] section is found |
1386 |
06 Sep 11 |
nicklas |
250 |
and then extract the SNP Count value and set that as the number of features |
1386 |
06 Sep 11 |
nicklas |
251 |
on the array design. |
1386 |
06 Sep 11 |
nicklas |
252 |
</description> |
1386 |
06 Sep 11 |
nicklas |
253 |
</about> |
1386 |
06 Sep 11 |
nicklas |
254 |
<index>1</index> |
1386 |
06 Sep 11 |
nicklas |
255 |
<action-factory> |
1386 |
06 Sep 11 |
nicklas |
256 |
<factory-class>net.sf.basedb.illumina.filehandler.SnpCvsValidationFactory</factory-class> |
1386 |
06 Sep 11 |
nicklas |
257 |
</action-factory> |
1386 |
06 Sep 11 |
nicklas |
258 |
</extension> |
1386 |
06 Sep 11 |
nicklas |
259 |
|
3477 |
03 Sep 15 |
jari |
260 |
</extensions> |